GeneTex
United States (US)

PPAT antibody

GTX102725_40618_WB_19011506_938.jpg
GTX102725_40618_IFA_19011506_286.jpg

Cat No. GTX102725

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ATASE antibody, GPAT antibody, PRAT antibody, PPAT antibody, amidophosphoribosyltransferase antibody, glutamine phosphoribosylpyrophosphatate amidotransferase antibody, glutamine phosphoribosylpyrophosphate amidotransferase antibody, glutamine PRPP amidotransferase antibody, phosphoribosyl pyrophosphate amidotransferase antibody

Background

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX102725_40618_WB_19011506_938.jpg

GTX102725 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX102725 diluted at 1:1000

GTX102725_40618_IFA_19011506_286.jpg

GTX102725 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PPAT(GTX102725) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf