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PPAT antibody

Anti-PPAT antibody used in Western Blot (WB). GTX102725
Anti-PPAT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102725

Cat No. GTX102725

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178730

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoribosyl pyrophosphate amidotransferase , ATASE , GPAT , PRAT

Background

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPAT antibody used in Western Blot (WB). GTX102725

GTX102725 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX102725 diluted at 1:1000

Anti-PPAT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102725

GTX102725 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PPAT(GTX102725) antibody at 1:500 dilution.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169