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PPEF1 antibody

Anti-PPEF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114652
Anti-PPEF1 antibody used in Western Blot (WB). GTX114652

Cat. No. GTX114652

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PPEF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

IMR32

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPEF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170478

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase with EF-hand domain 1 , PP7 , PPEF , PPP7C , PPP7CA

Background

This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPEF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114652

GTX114652 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PPEF1(GTX114652) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPEF1 antibody used in Western Blot (WB). GTX114652

GTX114652 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX114652 diluted at 1:10000

REFERENCE

Application Reference

Ye T et al. Onco Targets Ther 2020; 13 : 199-214 The Clinical Significance of PPEF1 as a Promising Biomarker and Its Potential Mechanism in Breast Cancer.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169