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PPIL4 antibody

Anti-PPIL4 antibody used in Western Blot (WB). GTX117014
Anti-PPIL4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117014

Cat No. GTX117014

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Mouse, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPIL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169136

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

peptidylprolyl isomerase like 4 , HDCME13P

Cellular Localization

Nucleus

Background

This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPIL4 antibody used in Western Blot (WB). GTX117014

GTX117014 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HepG2
C: HCT116
7.5% SDS PAGE
GTX117014 diluted at 1:1000

Anti-PPIL4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117014

GTX117014 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPIL4(GTX117014) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169