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PPM1A antibody [p6c7]

Anti-PPM1A antibody [p6c7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53766
Anti-PPM1A antibody [p6c7] used in Western Blot (WB). GTX53766
Anti-PPM1A antibody [p6c7] used in Western Blot (WB). GTX53766

Cat. No. GTX53766

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 p6c7

Isotype

 IgG2b

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($339)
See all PPM1A products

APPLICATION

Application Note

ELISA: Assay-dependent dilution
WB: 1:250- 1:1000
ICC/IF: 1:100
Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PP2C alpha (1-382aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase, Mg2+/Mn2+ dependent 1A , PP2C-ALPHA , PP2CA , PP2Calpha

Cellular Localization

Nucleus , Cytoplasm, cytosol , Membrane

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPM1A antibody [p6c7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53766

GTX53766 ICC/IF Image

PPM1A antibody detects PPM1A protein in HeLa cells by immunofluorescent analysis.
PPM1A antibody (GTX53766) dilution: 1:100

Anti-PPM1A antibody [p6c7] used in Western Blot (WB). GTX53766

GTX53766 WB Image

PPM1A antibody detects PPM1A protein by western blot analysis.
1. 40 μg Jurkat cell lysate
2. 40 μg HeLa cell lysate
3. 40 μg K562 cell lysate
4. 40 μg MCF7 cell lysate
5. 40 μg A549 cell lysate
6. 40 μg Raji cell lysate
PPM1A antibody (GTX53766) dilution: 1:1000

Anti-PPM1A antibody [p6c7] used in Western Blot (WB). GTX53766

GTX53766 WB Image

PPM1A antibody detects PPM1A protein by western blot analysis.
1. 40 μg Mouse kidney tissue lysate
2. 40 μg Mouse brain tissue lysate
3. 40 μg Mouse liver tissue lysate
PPM1A antibody (GTX53766) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339