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PPM1A antibody

Anti-PPM1A antibody used in Western Blot (WB). GTX109744
Anti-PPM1A antibody used in Western Blot (WB). GTX109744
Anti-PPM1A antibody used in Western Blot (WB). GTX109744
Anti-PPM1A antibody used in IHC (Paraffin sections) (IHC-P). GTX109744
Anti-PPM1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109744

Cat. No. GTX109744

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PPM1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Mouse brain , Raji , PC-12 , Rat2

Predict Reactivity

Rabbit, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPM1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951341

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase, Mg2+/Mn2+ dependent 1A , PP2C-ALPHA , PP2CA , PP2Calpha

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPM1A antibody used in Western Blot (WB). GTX109744

GTX109744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPM1A antibody (GTX109744) diluted at 1:500.

Anti-PPM1A antibody used in Western Blot (WB). GTX109744

GTX109744 WB Image

Sample (30 ug of whole cell lysate)
A: Raji (GTX27908)
10% SDS PAGE
GTX109744 diluted at 1:1000

Anti-PPM1A antibody used in Western Blot (WB). GTX109744

GTX109744 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX109744 diluted at 1:10000

Anti-PPM1A antibody used in IHC (Paraffin sections) (IHC-P). GTX109744

GTX109744 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPM1A(GTX109744) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPM1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109744

GTX109744 ICC/IF Image

PPM1A antibody detects PPM1A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PPM1A protein stained by PPM1A antibody (GTX109744) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Kikuchi R et al. FEBS Open Bio 2021; (Epub) Fenofibrate inhibits TGF-β-induced myofibroblast differentiation and activation in human lung fibroblasts in?vitro.
Application : WB
Reactivity : Human
Lu X et al. Oncogene 2014; 33 (22) : 2918-27 PPM1A is a RelA phosphatase with tumor suppressor-like activity.
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169