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United States (US)

PPM1A antibody

Anti-PPM1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33423
Anti-PPM1A antibody used in Western Blot (WB). GTX33423

Cat No. GTX33423

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

42 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human PPM1A (NP_066283.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Phosphatase, Mg2+/Mn2+ Dependent 1A,Pp2C-Alpha,Pp2Ca,Pp2Calpha,Ppm1A

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-PPM1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33423

GTX33423 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX33423 PPM1A antibody.
Blue : DAPI

Anti-PPM1A antibody used in Western Blot (WB). GTX33423

GTX33423 WB Image

WB analysis of various samples using GTX33423 PPM1A antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329