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PPM1G antibody [k1G6]

Anti-PPM1G antibody [k1G6] used in Western Blot (WB). GTX50041
Anti-PPM1G antibody [k1G6] used in IHC (Paraffin sections) (IHC-P). GTX50041

Cat. No. GTX50041

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 k1G6

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all PPM1G products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western blot is 1:1,000~1:2,000
Immunohistochemistry: Use at 1:50~100.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PPM1G (317-546aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase, Mg2+/Mn2+ dependent 1G , PP2CG , PP2CGAMMA , PPP2CG

Cellular Localization

Cytoplasm , Membrane

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-PPM1G antibody [k1G6] used in Western Blot (WB). GTX50041

GTX50041 WB Image

Cell lysates of 293T(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-PPM1G antibody [k1G6] used in IHC (Paraffin sections) (IHC-P). GTX50041

GTX50041 IHC-P Image

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human PPM1G (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399