GeneTex
United States (US)

PPM1K antibody [N1C3]

GTX105394_IHC_19011506_105.jpg
GTX105394_WB_19011506_774.jpg

Cat No. GTX105394

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

PPM1K tansfected 293T cell

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPM1K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp667B084 antibody, DKFZp761G058 antibody, PP2Ckappa antibody, PP2Cm antibody, PTMP antibody, UG0882E07 antibody, hPTMP antibody, PPM1K antibody, protein phosphatase 2C kappa antibody, PP2C-type mitochondrial phosphoprotein phosphatase antibody, "protein phosphatase 1K, mitochondrial antibody", PP2C domain-containing protein phosphatase 1K antibody, PP2C-kappa antibody, protein phosphatase 2C isoform kappa antibody, PP2C-like mitochondrial protein antibody, PP2C-like protein antibody, protein phosphatase 1K (PP2C domain containing) antibody, PP2C type mitochondrial phosphatase antibody, "protein phosphatase, Mg2+/Mn2+ dependent, 1K antibody"

Cellular Localization

Mitochondrion matrix

Background

Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development.
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Database

Research Area

DATA IMAGES
GTX105394_IHC_19011506_105.jpg

GTX105394 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using PPM1K(GTX105394) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105394_WB_19011506_774.jpg

GTX105394 WB Image

Western Blot analysis of PPM1K expression in transfected 293T cell line by PPM1K polyclonal antibody.
A: Non-transfected lysate.
B: PPM1K transfected lysate.
12% SDS PAGE
GTX105394 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf