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PPME1 antibody [7F12]

Overexpression
Anti-PPME1 antibody [7F12] used in Flow cytometry (FACS). GTX83847
Anti-PPME1 antibody [7F12] used in Flow cytometry (FACS). GTX83847
Anti-PPME1 antibody [7F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83847
Anti-PPME1 antibody [7F12] used in Western Blot (WB). GTX83847
Anti-PPME1 antibody [7F12] used in Western Blot (WB). GTX83847
Anti-PPME1 antibody [7F12] used in IHC (Paraffin sections) (IHC-P). GTX83847

Cat. No. GTX83847

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7F12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all PPME1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase methylesterase 1 , ABDH19 , PME-1

Background

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro).

Database

Research Area

DATA IMAGES

Anti-PPME1 antibody [7F12] used in Flow cytometry (FACS). GTX83847

GTX83847 FACS Image

FACS analysis of Jurkat cells using GTX83847 PPME1 antibody [7F12].
Red : Primary antibody
Blue : Negative control antibody

Anti-PPME1 antibody [7F12] used in Flow cytometry (FACS). GTX83847

GTX83847 FACS Image

FACS analysis of HeLa cells using GTX83847 PPME1 antibody [7F12].
Red : Primary antibody
Blue : Negative control antibody

Anti-PPME1 antibody [7F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83847

GTX83847 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with PPME1 plasmid using GTX83847 PPME1 antibody [7F12].

Anti-PPME1 antibody [7F12] used in Western Blot (WB). GTX83847

GTX83847 WB Image

WB analysis of various cell lines using GTX83847 PPME1 antibody [7F12].
Loading : 35 ug per lane
Dilution : 1:200

Anti-PPME1 antibody [7F12] used in Western Blot (WB). GTX83847

GTX83847 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PPME1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83847 PPME1 antibody [7F12].
Loading : 5 ug per lane

Anti-PPME1 antibody [7F12] used in IHC (Paraffin sections) (IHC-P). GTX83847

GTX83847 IHC-P Image

IHC-P analysis of kidney tissue using GTX83847 PPME1 antibody [7F12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519