GeneTex
United States (US)

PPME1 antibody

GTX102660_40877_IHC_19011506_676.jpg
GTX102660_40877_WB_M_19011506_461.jpg
GTX102660_40877_WB_19011506_977.jpg

Cat No. GTX102660

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPME1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ22226 antibody, PME-1 antibody, PPME1 antibody, protein phosphatase methylesterase 1 antibody, protein phosphatase methylesterase-1 antibody

Background

Protein phosphatase methylesterase-1 catalyzes the demethylation of the protein phosphatase-2A catalytic subunit (PPP2CA; MIM 176915) (Ogris et al., 1999 [PubMed 10318862]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102660_40877_IHC_19011506_676.jpg

GTX102660 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PME-1(GTX102660) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102660_40877_WB_M_19011506_461.jpg

GTX102660 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX102660 diluted at 1:1000

GTX102660_40877_WB_19011506_977.jpg

GTX102660 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX102660 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169