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PPOX antibody

Anti-PPOX antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33424
Anti-PPOX antibody used in Western Blot (WB). GTX33424
Anti-PPOX antibody used in IHC (Paraffin sections) (IHC-P). GTX33424

Cat. No. GTX33424

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PPOX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PPOX (NP_001116236.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protoporphyrinogen oxidase , PPO , V290M , VP

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPOX antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33424

GTX33424 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX33424 PPOX antibody.
Blue : DAPI

Anti-PPOX antibody used in Western Blot (WB). GTX33424

GTX33424 WB Image

WB analysis of various sample lysates using GTX33424 PPOX antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PPOX antibody used in IHC (Paraffin sections) (IHC-P). GTX33424

GTX33424 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX33424 PPOX antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399