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PPP1A antibody [1C11-C10-H5-E9]

Anti-PPP1A antibody [1C11-C10-H5-E9] used in IHC (Paraffin sections) (IHC-P). GTX49238
Anti-PPP1A antibody [1C11-C10-H5-E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49238
Anti-PPP1A antibody [1C11-C10-H5-E9] used in Western Blot (WB). GTX49238

Cat No. GTX49238

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1C11-C10-H5-E9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($319)
See all PPP1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human PPP1CA protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Protein Phosphatase 1 Catalytic Subunit Alpha , Pp-1A , Pp1A , Pp1Alpha , Ppp1A , Ppp1Ca

Cellular Localization

Cytoplasm. Nucleus. Nucleus

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPP1A antibody [1C11-C10-H5-E9] used in IHC (Paraffin sections) (IHC-P). GTX49238

GTX49238 IHC-P Image

IHC-P analysis of human breast cancer using PPP1A antibody.

Anti-PPP1A antibody [1C11-C10-H5-E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49238

GTX49238 ICC/IF Image

ICC/IF analysis of HeLa cells using PPP1A antibody at a dilution of 1:300.

Anti-PPP1A antibody [1C11-C10-H5-E9] used in Western Blot (WB). GTX49238

GTX49238 WB Image

WB analysis of A431, Jurkat and HeLa lysates using PPP1A antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319