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PPP1A antibody [4G3]

Anti-PPP1A antibody [4G3] used in Western Blot (WB). GTX57557
Anti-PPP1A antibody [4G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57557

Cat. No. GTX57557

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G3

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all PPP1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 4G3 is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPP3CA protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 catalytic subunit alpha , PP1A , PP1alpha , PPP1A , PPP1CA , PP1 alpha

Cellular Localization

Cytoplasm,Nucleus,Nucleoplasm,Nucleolus

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPP1A antibody [4G3] used in Western Blot (WB). GTX57557

GTX57557 WB Image

WB analysis of HepG2 whole cell lysate using GTX57557 PPP1A antibody.
Loading : 45 μg
Dilution : 1:1000

Anti-PPP1A antibody [4G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57557

GTX57557 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57557 PPP1A antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399