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PPP1A antibody [5E9]

Anti-PPP1A antibody [5E9] used in Western Blot (WB). GTX60538
Anti-PPP1A antibody [5E9] used in ELISA (ELISA). GTX60538
Anti-PPP1A antibody [5E9] used in Western Blot (WB). GTX60538

Cat. No. GTX60538

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5E9

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all PPP1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ELISA 1/10000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PPP1CA expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 catalytic subunit alpha , PP-1A , PP1A , PP1alpha , PPP1A

Cellular Localization

Cytoplasm,Nucleus,nucleoplasm,Nucleolus

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPP1A antibody [5E9] used in Western Blot (WB). GTX60538

GTX60538 WB Image

WB analysis of A431 (1),  Hela (2),  HepG2 (3),  MCF-7 (4),  Jurkat (5)  and  A549 (6)  cells using GTX60538 PPP1A antibody [5E9].

Anti-PPP1A antibody [5E9] used in ELISA (ELISA). GTX60538

GTX60538 ELISA Image

ELISA analysis of antigen using GTX60538 PPP1A antibody [5E9].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-PPP1A antibody [5E9] used in Western Blot (WB). GTX60538

GTX60538 WB Image

WB analysis of human PPP1A (AA: 174-330) recombinant protein using GTX60538 PPP1A antibody [5E9].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409