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PPP1A antibody

Anti-PPP1A antibody used in Western Blot (WB). GTX105255
Anti-PPP1A antibody used in Western Blot (WB). GTX105255
Anti-PPP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX105255
Anti-PPP1A antibody used in Western Blot (WB). GTX105255
Anti-PPP1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105255

Cat No. GTX105255

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , rat brain

Predict Reactivity

Mouse, Rabbit, Bovine, Dog, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

protein phosphatase 1 catalytic subunit alpha , PP-1A , PP1A , PP1alpha , PPP1A

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PPP1A antibody used in Western Blot (WB). GTX105255

GTX105255 WB Image

WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using GTX105255 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP1A antibody used in Western Blot (WB). GTX105255

GTX105255 WB Image

PPP1A antibody detects PPP1A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX105255

GTX105255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A(GTX105255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPP1A antibody used in Western Blot (WB). GTX105255

GTX105255 WB Image

PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105255

GTX105255 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A(GTX105255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169