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PPP1A antibody

KO/KD
Anti-PPP1A antibody used in Western Blot (WB). GTX105255
Anti-PPP1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105255
Anti-PPP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX105255
Anti-PPP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX105255
Anti-PPP1A antibody used in Western Blot (WB). GTX105255
Anti-PPP1A antibody used in Western Blot (WB). GTX105255
Anti-PPP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX105255
Anti-PPP1A antibody used in Western Blot (WB). GTX105255

Cat. No. GTX105255

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PPP1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

rat brain

Product Note

Based on sequence homology, this antibody may cross react with PPP1CB or PPP1CC proteins.

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951346

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 catalytic subunit alpha , PP-1A , PP1A , PP1alpha , PPP1A

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPP1A antibody used in Western Blot (WB). GTX105255

GTX105255 WB Image

KO/KD

WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using GTX105255 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105255

GTX105255 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A(GTX105255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-PPP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX105255

GTX105255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A(GTX105255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PPP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX105255

GTX105255 IHC-P Image

PPP1A antibody detects PPP1A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
PPP1A stained by PPP1A antibody (GTX105255) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPP1A antibody used in Western Blot (WB). GTX105255

GTX105255 WB Image

PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP1A antibody used in Western Blot (WB). GTX105255

GTX105255 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted at 1:500.

Anti-PPP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX105255

GTX105255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using PPP1A antibody (GTX105255) at 1:300 dilution.

Anti-PPP1A antibody used in Western Blot (WB). GTX105255

GTX105255 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Yang J et al. Exp Ther Med 2023; 25 (4) : 154 KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma.
Application : WB, IP
Reactivity : Human
Noguchi Y et al. iScience 2021; 24 (7) : 102724 Microscopic image-based covariation network analysis for actin scaffold-modified insulin signaling.
Application : ICC/IF
Reactivity : Rat
Liu CY et al. Br J Haematol 2017; (Epub) Carfilzomib induces leukaemia cell apoptosis via inhibiting ELK1/KIAA1524 (Elk-1/CIP2A) and activating PP2A not related to proteasome inhibition.
Application : IP
Reactivity : Human
Wei R et al. Mol Biol Cell 2011; 22 (19) : 3584-94 Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169