GeneTex
United States (US)

PPP1A antibody

KO/KD
GTX105255_WB_GK_19011506_274.jpg
GTX105255_41017_WB_2_19011506_461.jpg
GTX105255_IHC_19011506_639.jpg
GTX105255_39932_WB_R_brain_19011506_612.jpg
GTX105255_39932_IFA_19011506_112.jpg

Cat No. GTX105255

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , rat brain

Predict Reactivity

Mouse, Rabbit, Bovine, Dog, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC15877 antibody, MGC1674 antibody, PP-1A antibody, PP1alpha antibody, PPP1A antibody, PPP1CA antibody, serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit antibody, "protein phosphatase 1, catalytic subunit, alpha isoform antibody", serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody, "protein phosphatase 1, catalytic subunit, alpha isozyme antibody"

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105255_WB_GK_19011506_274.jpg

GTX105255 WB Image

KO/KD

WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using GTX105255 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) GTX70220 at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105255_41017_WB_2_19011506_461.jpg

GTX105255 WB Image

PPP1A antibody detects PPP1A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105255_IHC_19011506_639.jpg

GTX105255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A(GTX105255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105255_39932_WB_R_brain_19011506_612.jpg

GTX105255 WB Image

PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody (GTX105255) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105255_39932_IFA_19011506_112.jpg

GTX105255 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A(GTX105255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf