GeneTex
United States (US)

PPP1CB antibody

GTX105295_40009_20181221_WB_shRNA_watermark_19011519_371.jpg
GTX105295_40009_WB_M_brain_19011506_102.jpg
GTX105295_43117_20180307_ICC_IF_19011506_894.jpg
GTX105295_40009_20141017_IHC_M_2_19011506_877.jpg
GTX105295_40009_20170518_WB_19011506_389.jpg
GTX105295_40009_20170831_IHC-Fr_M_19011506_254.jpg
GTX105295_40009_20141017_IHC_M_19011506_693.jpg

Cat No. GTX105295

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PPP1CB shRNA-transfected 293T , Mouse brain

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP1CB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC3672 antibody, PP-1B antibody, PP1beta antibody, PPP1CD antibody, PPP1CB antibody, protein phosphatase 1-beta antibody, protein phosphatase 1-delta antibody, "protein phosphatase 1, catalytic subunit, delta isoform antibody", serine/threonine protein phosphatase PP1-beta catalytic subunit antibody, "protein phosphatase 1, catalytic subunit, beta isoform antibody", serine/threonine-protein phosphatase PP1-beta catalytic subunit antibody, "protein phosphatase 1, catalytic subunit, beta isozyme antibody"

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105295_40009_20181221_WB_shRNA_watermark_19011519_371.jpg

GTX105295 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody (GTX105295) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105295_40009_WB_M_brain_19011506_102.jpg

GTX105295 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX105295 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105295_43117_20180307_ICC_IF_19011506_894.jpg

GTX105295 ICC/IF Image

PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105295_40009_20141017_IHC_M_2_19011506_877.jpg

GTX105295 IHC-P Image

PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
PPP1CB antibody (GTX105295) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105295_40009_20170518_WB_19011506_389.jpg

GTX105295 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody (GTX105295) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105295_40009_20170831_IHC-Fr_M_19011506_254.jpg

GTX105295 IHC-Fr Image

PPP1CB antibody detects PPP1CB Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX105295_40009_20141017_IHC_M_19011506_693.jpg

GTX105295 IHC-P Image

PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
PPP1CB antibody (GTX105295) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169