APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , 293T
Product Note
Based on sequence homology, this antibody may cross react with PPP1CA or PPP1CB proteins.
Predict Reactivity
Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PPP1CB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951348
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein phosphatase 1 catalytic subunit beta , HEL-S-80p , NSLH2 , PP-1B , PP1B , PP1beta , PPP1CD
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105295 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody (GTX105295) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105295 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody (GTX105295) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105295 IHC-Fr Image
PPP1CB antibody detects PPP1CB Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX105295 ICC/IF Image
PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:500.Blue: Hoechst 33342 staining.
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GTX105295 WB Image
Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX105295 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105295 IHC-P Image
PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
PPP1CB antibody (GTX105295) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX105295 IHC-P Image
PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105295 IHC-P Image
PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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