GeneTex
  • Country / Location Selection

United States (US)

PPP1CB antibody

Anti-PPP1CB antibody used in Western Blot (WB). GTX105295
Anti-PPP1CB antibody used in Western Blot (WB). GTX105295
Anti-PPP1CB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105295
Anti-PPP1CB antibody used in IHC (Frozen sections) (IHC-Fr). GTX105295
Anti-PPP1CB antibody used in IHC (Paraffin sections) (IHC-P). GTX105295
Anti-PPP1CB antibody used in IHC (Paraffin sections) (IHC-P). GTX105295
Anti-PPP1CB antibody used in Western Blot (WB). GTX105295
Anti-PPP1CB antibody used in IHC (Paraffin sections) (IHC-P). GTX105295

Cat. No. GTX105295

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Mouse brain , 293T

Product Note

Based on sequence homology, this antibody may cross react with PPP1CA or PPP1CB proteins.

Predict Reactivity

Zebrafish, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP1CB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951348

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 1 catalytic subunit beta , HEL-S-80p , NSLH2 , PP-1B , PP1B , PP1beta , PPP1CD

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPP1CB antibody used in Western Blot (WB). GTX105295

GTX105295 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody (GTX105295) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP1CB antibody used in Western Blot (WB). GTX105295

GTX105295 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX105295 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP1CB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105295

GTX105295 ICC/IF Image

PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PPP1CB antibody used in IHC (Frozen sections) (IHC-Fr). GTX105295

GTX105295 IHC-Fr Image

PPP1CB antibody detects PPP1CB Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-PPP1CB antibody used in IHC (Paraffin sections) (IHC-P). GTX105295

GTX105295 IHC-P Image

PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:3000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPP1CB antibody used in IHC (Paraffin sections) (IHC-P). GTX105295

GTX105295 IHC-P Image

PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PPP1CB stained by PPP1CB antibody (GTX105295) diluted at 1:3000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPP1CB antibody used in Western Blot (WB). GTX105295

GTX105295 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP1CB antibody (GTX105295) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP1CB antibody used in IHC (Paraffin sections) (IHC-P). GTX105295

GTX105295 IHC-P Image

PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
PPP1CB antibody (GTX105295) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for PPP1CB antibody (GTX105295). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PPP1CB antibody (GTX105295). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169