PPP2R1A antibody

Name: PPP2R1A antibody
Brand: GeneTex
SKU: GTX102206
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-PPP2R1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102206

Anti-PPP2R1A antibody used in Western Blot (WB). GTX102206

Anti-PPP2R1A antibody used in IHC (Paraffin sections) (IHC-P). GTX102206

Cat. No. GTX102206

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all PPP2R1A products

View product citations for antibody GTX102206 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:2500
IP	Assay dependent

Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , HeLa

Predict Reactivity

Rat, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP2R1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 2 scaffold subunit Aalpha , MRD36 , PP2A-Aalpha , PP2AA , PP2AAALPHA , PR65A

Background

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq]

Database

Gene ID: 5518 PPP2R1A
UniProt: P30153 PPP2R1A

Research Area

DATA IMAGES

GTX102206 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PPP2R1A(GTX102206) antibody at 1:500 dilution.

GTX102206 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX102206 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102206 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP2R1A antibody (GTX102206) diluted at 1:1000.

GTX102206 IHC-P Image

PPP2R1A antibody detects PPP2R1A protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PPP2R1A antibody (GTX102206) diluted at 1:2500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yu-Ting Yen et al. Nat Commun 2021; 12 (1) : 7297 Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.

Application : WB, IHC-P, IP

Reactivity : Mouse

Yen Y.T. et al. Commun Biol. 2021; 4 (1) : 658 PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation.

Application : WB, IHC-P

Reactivity : Mouse

Yen YT et al. Cells 2020; 9 (1) : pii: E90 PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo.

Application : IHC-P

Reactivity : Mouse

Yen YT et al. Cells 2019; 9 (1) PP2A Deficiency Enhances Carcinogenesis of Lgr5⁺ Intestinal Stem Cells Both in Organoids and In Vivo.

Application : ICC/IF

Reactivity : Mouse

Adhikari B et al. Biochim Biophys Acta Mol Basis Dis 2019; 1865 (2) : 322-328 Neuronal ceroid lipofuscinosis related ER membrane protein CLN8 regulates PP2A activity and ceramide levels.

Application : WB

Reactivity : Human

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