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PPP2R1A antibody

Anti-PPP2R1A antibody used in IHC (Paraffin sections) (IHC-P). GTX102206
Anti-PPP2R1A antibody used in Western Blot (WB). GTX102206
Anti-PPP2R1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102206
Anti-PPP2R1A antibody used in Western Blot (WB). GTX102206

Cat No. GTX102206

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:2500
IP Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HeLa , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP2R1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 2 scaffold subunit Aalpha , MRD36 , PP2A-Aalpha , PP2AA , PP2AAALPHA , PR65A

Background

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PPP2R1A antibody used in IHC (Paraffin sections) (IHC-P). GTX102206

GTX102206 IHC-P Image

PPP2R1A antibody detects PPP2R1A protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PPP2R1A antibody (GTX102206) diluted at 1:2500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPP2R1A antibody used in Western Blot (WB). GTX102206

GTX102206 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX102206 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP2R1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102206

GTX102206 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PPP2R1A(GTX102206) antibody at 1:500 dilution.

Anti-PPP2R1A antibody used in Western Blot (WB). GTX102206

GTX102206 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP2R1A antibody (GTX102206) diluted at 1:1000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169