GeneTex
United States (US)

PPP2R1A antibody

GTX102206_42347_20160302_IHC-P_M_19011506_950.jpg
GTX102206_40499_WB_M_19011506_412.jpg
GTX102206_40499_IFA_19011506_358.jpg
GTX102206_42347_20160107_WB_19011506_394.jpg

Cat No. GTX102206

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:2500
IP Assay dependent
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HeLa , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP2R1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC786 antibody, PP2A-Aalpha antibody, PP2AAALPHA antibody, PR65A antibody, PPP2R1A antibody, PP2A subunit A isoform PR65-alpha antibody, medium tumor antigen-associated 61 KDA protein antibody, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody, PP2A subunit A isoform R1-alpha antibody, "protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform antibody", "serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform antibody", "protein phosphatase 2, regulatory subunit A, alpha antibody"

Background

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102206_42347_20160302_IHC-P_M_19011506_950.jpg

GTX102206 IHC-P Image

PPP2R1A antibody detects PPP2R1A protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PPP2R1A antibody (GTX102206) diluted at 1:2500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102206_40499_WB_M_19011506_412.jpg

GTX102206 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX102206 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102206_40499_IFA_19011506_358.jpg

GTX102206 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PPP2R1A(GTX102206) antibody at 1:500 dilution.

GTX102206_42347_20160107_WB_19011506_394.jpg

GTX102206 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP2R1A antibody (GTX102206) diluted at 1:1000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169