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PPP2R2D antibody [N2C3]

KO/KD Orthogonal
Anti-PPP2R2D antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116609
Anti-PPP2R2D antibody [N2C3] used in Western Blot (WB). GTX116609
Anti-PPP2R2D antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116609
Anti-PPP2R2D antibody [N2C3] used in Western Blot (WB). GTX116609
Anti-PPP2R2D antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116609

Cat. No. GTX116609

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PPP2R2D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

IMR32 , mouse brain , rat brain

Predict Reactivity

Zebrafish, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP2R2D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615217

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 2 regulatory subunit Bdelta , B55D , B55delta , MDS026

Cellular Localization

Cytoplasm

Background

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Database

Research Area

DATA IMAGES

Anti-PPP2R2D antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116609

GTX116609 IHC-P Image

PPP2R2D antibody [N2C3] detects PPP2R2D protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPP2R2D antibody [N2C3] used in Western Blot (WB). GTX116609

GTX116609 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP2R2D antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116609

GTX116609 IHC-P Image

PPP2R2D antibody [N2C3] detects PPP2R2D protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPP2R2D antibody [N2C3] used in Western Blot (WB). GTX116609

GTX116609 WB Image

Sample (30 μg of whole cell lysate)
A:IMR32
10% SDS PAGE
GTX116609 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PPP2R2D antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX116609

GTX116609 IHC-P Image

PPP2R2D antibody [N2C3] detects PPP2R2D protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PPP2R2D antibody [N2C3] (GTX116609) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kim YS et al. Nat Commun 2022; 13 (1) : 6080 Induction of the hepatic aryl hydrocarbon receptor by alcohol dysregulates autophagy and phospholipid metabolism via PPP2R2D.
Application : WB, IHC-P
Reactivity : Human, Mouse
Alvarez-Fernandez M et al. Cell Death Differ 2017; (Epub) Therapeutic relevance of the PP2A-B55 inhibitory kinase MASTL/Greatwall in breast cancer.
Application : IHC-P
Reactivity : Human
Joseph BK et al. J Biol Chem 2015; 290 (17) : 10588-98 Inhibition of AMP Kinase by the Protein Phosphatase 2A Heterotrimer, PP2APpp2r2d.
Application : WB
Reactivity : Rat
Kurimchak A et al. Mol Cell Biol 2013; 33 (16) : 3330-42 Activation of p107 by fibroblast growth factor, which is essential for chondrocyte cell cycle exit, is mediated by the protein phosphatase 2A/B55α holoenzyme.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169