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PPP2R4 antibody [4D9]

Anti-PPP2R4 antibody [4D9] used in ELISA (ELISA). GTX60539
Anti-PPP2R4 antibody [4D9] used in IHC (Paraffin sections) (IHC-P). GTX60539
Anti-PPP2R4 antibody [4D9] used in IHC (Paraffin sections) (IHC-P). GTX60539
Anti-PPP2R4 antibody [4D9] used in Western Blot (WB). GTX60539
Anti-PPP2R4 antibody [4D9] used in Flow cytometry (FACS). GTX60539
Anti-PPP2R4 antibody [4D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60539

Cat. No. GTX60539

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4D9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all PPP2R4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PPP2R4 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 2 phosphatase activator , PP2A , PPP2R4 , PR53

Cellular Localization

Cytoplasm,Nucleus

Background

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPP2R4 antibody [4D9] used in ELISA (ELISA). GTX60539

GTX60539 ELISA Image

ELISA analysis of antigen using GTX60539 PPP2R4 antibody [4D9].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-PPP2R4 antibody [4D9] used in IHC (Paraffin sections) (IHC-P). GTX60539

GTX60539 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60539 PPP2R4 antibody [4D9].

Anti-PPP2R4 antibody [4D9] used in IHC (Paraffin sections) (IHC-P). GTX60539

GTX60539 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60539 PPP2R4 antibody [4D9].

Anti-PPP2R4 antibody [4D9] used in Western Blot (WB). GTX60539

GTX60539 WB Image

WB analysis of human PPP2R4 (AA: 1-154) recombinant protein using GTX60539 PPP2R4 antibody [4D9].

Anti-PPP2R4 antibody [4D9] used in Flow cytometry (FACS). GTX60539

GTX60539 FACS Image

FACS analysis of MCF-7 cells using GTX60539 PPP2R4 antibody [4D9].
Green : PPP2R4
Red : negative control

Anti-PPP2R4 antibody [4D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60539

GTX60539 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60539 PPP2R4 antibody [4D9].
Green : PPP2R4
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409