GeneTex
United States (US)

PPP2R4 antibody, C-term

GTX88415_2350_WB_18121411_581.jpg
GTX88415_970_IHC-P_18121411_737.jpg

Cat No. GTX88415

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-2μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88415-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-GSLLPIHPVTSG, from the C Terminus of the protein sequence according to NP_821068.1; NP_066954.2; NP_821070.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Phosphatase 2 Phosphatase Activator,Pp2A,Ppp2R4,Pr53,Ptpa

Background

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88415_2350_WB_18121411_581.jpg

GTX88415 WB Image

WB analysis of human liver lysate (lane 1), mouse liver (lane 2) and rat liver (lane 3) using GTX88415 PPP2R4 antibody, C-term.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

GTX88415_970_IHC-P_18121411_737.jpg

GTX88415 IHC-P Image

IHC-P analysis of human testis using GTX88415 PPP2R4 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

Package List Price ($)
$ 319