GeneTex
United States (US)

PPP2R5D antibody [C3], C-term

KO/KD
GTX106401_WB_19011506_669.jpg
GTX106401_39694_IHC_19011506_442.jpg
GTX106401_39694_20180810_WB_shRNA_watermark_19011506_370.jpg

Cat No. GTX106401

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

HeLa , A549 , IMR32 , HCT116 , PPP2R5D shRNA-transfected HeLa

Predict Reactivity

Mouse, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PPP2R5D. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B56D antibody, MGC2134 antibody, MGC8949 antibody, PPP2R5D antibody, "PP2A, B subunit, R5 delta isoform antibody", serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform antibody, "protein phosphatase 2, regulatory subunit B', delta isoform antibody", PP2A B subunit isoform B56-delta antibody, "PP2A, B subunit, B56 delta isoform antibody", "PP2A, B subunit, B' delta isoform antibody", "PP2A, B subunit, PR61 delta isoform antibody", PP2A B subunit isoform R5-delta antibody, "Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform antibody", PP2A B subunit isoform PR61-delta antibody, "protein phosphatase 2, regulatory subunit B (B56), delta isoform antibody", PP2A B subunit isoform B'-delta antibody, "protein phosphatase 2, regulatory subunit B', delta antibody"

Cellular Localization

Cytoplasm , Nucleus

Background

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
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Database

DATA IMAGES
GTX106401_WB_19011506_669.jpg

GTX106401 WB Image

Sample (30 ug of whole cell lysate)
A: A549 B: IMR32 7.5% SDS PAGE
GTX106401 diluted at 1:1000

GTX106401_39694_IHC_19011506_442.jpg

GTX106401 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human tyroid cancer, using PPP2R5D(GTX106401) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106401_39694_20180810_WB_shRNA_watermark_19011506_370.jpg

GTX106401 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP2R5D antibody [C3], C-term (GTX106401) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169