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PPP3CB antibody [N1N3]

KO/KD Overexpression
Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141
Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141
Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141
Anti-PPP3CB antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111141
Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

Cat. No. GTX111141

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PPP3CB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Mouse brain , Molt-4 , HeLa , 293T , PPP3CB-transfected 293T

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPP3CB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951361

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 3 catalytic subunit beta , CALNA2 , CALNB , CNA2 , PP2Bbeta

Background

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Database

Research Area

DATA IMAGES

Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

GTX111141 WB Image

KO/KD

Wild-type (WT) and TLE1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PPP3CB antibody [N1N3] (GTX111141) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

GTX111141 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111141 diluted at 1:5000

Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

GTX111141 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111141 diluted at 1:1000

Anti-PPP3CB antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX111141

GTX111141 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using PPP3CB(GTX111141) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PPP3CB antibody [N1N3] used in Western Blot (WB). GTX111141

GTX111141 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP3CB antibody [N1N3] (GTX111141) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169