PPP4C antibody [N1], N-term

Cat. No. GTX108299

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all PPP4C products

Datasheet
Document

View product citations for antibody GTX108299 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431 , HeLaS3

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PPP4C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951366

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 4 catalytic subunit , PP-X , PP4 , PP4C , PPH3 , PPP4 , PPX

Cellular Localization

Cytoplasm , Nucleus , Centrosome

Background

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase.

Database

Gene ID: 5531 PPP4C
UniProt: P60510 PPP4C

Research Area

DATA IMAGES

GTX108299 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPP4C(GTX108299) antibody at 1:500 dilution.

GTX108299 IHC-P Image

PPP4C antibody [N1], N-term detects PPP4C protein at nucleus and cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
PPP4C antibody [N1], N-term (GTX108299) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108299 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX108299 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty