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PPP5C antibody [2E12]

Overexpression
Anti-PPP5C antibody [2E12] used in Western Blot (WB). GTX83832
Anti-PPP5C antibody [2E12] used in IHC (Paraffin sections) (IHC-P). GTX83832
Anti-PPP5C antibody [2E12] used in Flow cytometry (FACS). GTX83832
Anti-PPP5C antibody [2E12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83832
Anti-PPP5C antibody [2E12] used in IHC (Paraffin sections) (IHC-P). GTX83832
Anti-PPP5C antibody [2E12] used in IHC (Paraffin sections) (IHC-P). GTX83832
Anti-PPP5C antibody [2E12] used in Western Blot (WB). GTX83832

Cat. No. GTX83832

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2E12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all PPP5C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human PPP5C (NP_006238) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 5 catalytic subunit , PP5 , PPP5 , PPT

Cellular Localization

Nucleus,Cytoplasm,Cell membrane

Background

May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Database

Research Area

DATA IMAGES

Anti-PPP5C antibody [2E12] used in Western Blot (WB). GTX83832

GTX83832 WB Image

Overexpression

WB analysis of HEK293T cells transfected with PPP5C plasmid (Right) or empty vector (Left) for 48 hrs using GTX83832 PPP5C antibody [2E12].
Loading : 5 ug per lane

Anti-PPP5C antibody [2E12] used in IHC (Paraffin sections) (IHC-P). GTX83832

GTX83832 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX83832 PPP5C antibody [2E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PPP5C antibody [2E12] used in Flow cytometry (FACS). GTX83832

GTX83832 FACS Image

FACS analysis of HEK293T cells transfected with either PPP5C plasmid(Red) or empty vector control plasmid(Blue) using GTX83832 PPP5C antibody [2E12].

Anti-PPP5C antibody [2E12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83832

GTX83832 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83832 PPP5C antibody [2E12].

Anti-PPP5C antibody [2E12] used in IHC (Paraffin sections) (IHC-P). GTX83832

GTX83832 IHC-P Image

IHC-P analysis of human ovary tissue using GTX83832 PPP5C antibody [2E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PPP5C antibody [2E12] used in IHC (Paraffin sections) (IHC-P). GTX83832

GTX83832 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83832 PPP5C antibody [2E12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-PPP5C antibody [2E12] used in Western Blot (WB). GTX83832

GTX83832 WB Image

WB analysis of various cell lines using GTX83832 PPP5C antibody [2E12].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519