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PPWD1 antibody

Anti-PPWD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123517
Anti-PPWD1 antibody used in Western Blot (WB). GTX123517
Anti-PPWD1 antibody used in Western Blot (WB). GTX123517

Cat. No. GTX123517

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PPWD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

293T , Rat bone marrow

Predict Reactivity

Mouse, Zebrafish, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPWD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171775

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidylprolyl isomerase domain and WD repeat containing 1

Background

Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.

Database

DATA IMAGES

Anti-PPWD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123517

GTX123517 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPWD1(GTX123517) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-PPWD1 antibody used in Western Blot (WB). GTX123517

GTX123517 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX123517 diluted at 1:2000

Anti-PPWD1 antibody used in Western Blot (WB). GTX123517

GTX123517 WB Image

Sample (50 ug of whole cell lysate)
A: Rat bone marrow
7.5% SDS PAGE
GTX123517 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169