GeneTex
United States (US)

PPWD1 antibody

GTX123517_40744_IFA_18121410_496.jpg
GTX123517_40744_WB_R_Bone marrow_18121410_814.jpg
GTX123517_40744_WB_18121410_634.jpg

Cat No. GTX123517

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa kDa. ( Note )

Positive Control

K562 , THP-1 , Rat bone marrow

Predict Reactivity

Mouse, Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PPWD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

KIAA0073 antibody, PPWD1 antibody, peptidylprolyl isomerase domain and WD repeat-containing protein 1 antibody, spliceosome-associated cyclophilin antibody, peptidylprolyl isomerase domain and WD repeat containing 1 antibody

Background

Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing.
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Database

DATA IMAGES
GTX123517_40744_IFA_18121410_496.jpg

GTX123517 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPWD1(GTX123517) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX123517_40744_WB_R_Bone marrow_18121410_814.jpg

GTX123517 WB Image

Sample (50 ug of whole cell lysate)
A: Rat bone marrow
7.5% SDS PAGE
GTX123517 diluted at 1:1000

GTX123517_40744_WB_18121410_634.jpg

GTX123517 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX123517 diluted at 1:2000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169