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PRAF1 antibody [C1C3]

Anti-PRAF1 antibody [C1C3] used in Western Blot (WB). GTX116763
Anti-PRAF1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116763

Cat No. GTX116763

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

A549 , HepG2 , HCT116

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRAF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615660

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

RNA polymerase I subunit E , PAF53 , PRAF1

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.

Database

DATA IMAGES

Anti-PRAF1 antibody [C1C3] used in Western Blot (WB). GTX116763

GTX116763 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX116763 diluted at 1:1000

Anti-PRAF1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX116763

GTX116763 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using PRAF1(GTX116763) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for PRAF1 antibody [C1C3] (GTX116763). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PRAF1 antibody [C1C3] (GTX116763). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169