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PRAK antibody [7H10B4]

Anti-PRAK antibody [7H10B4] used in IHC (Paraffin sections) (IHC-P). GTX83275
Anti-PRAK antibody [7H10B4] used in Western Blot (WB). GTX83275

Cat. No. GTX83275

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7H10B4

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all PRAK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of PRAK expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase-activated protein kinase 5 , MAPKAP-K5 , MK-5 , MK5 , PRAK

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-PRAK antibody [7H10B4] used in IHC (Paraffin sections) (IHC-P). GTX83275

GTX83275 IHC-P Image

IHC-P analysis of human liver carcinoma (A), esophagus carcinoma (B), normal spleen tissue(C), breast carcinoma (D) using GTX83275 PRAK antibody [7H10B4].

Anti-PRAK antibody [7H10B4] used in Western Blot (WB). GTX83275

GTX83275 WB Image

WB analysis of truncated PRAK recombinant protein using GTX83275 PRAK antibody [7H10B4].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399