GeneTex
United States (US)

PRAK antibody [N1C2]

GTX108759_WB_19011507_668.jpg

Cat No. GTX108759

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PRAK antibody, MAPKAPK5 antibody, MAPK-activated protein kinase 5 antibody, MAPKAP kinase 5 antibody, MAP kinase-activated protein kinase 5 antibody, MAPKAPK-5 antibody, p38-regulated/activated protein kinase antibody, mitogen-activated protein kinase-activated protein kinase 5 antibody

Cellular Localization

Cytoplasm (Probable) , Nucleus

Background

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX108759_WB_19011507_668.jpg

GTX108759 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX108759 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf