GeneTex
United States (US)

PRAK antibody [N3C3]

GTX107938_IFA_19011507_508.jpg
GTX107938_WB_19011507_646.jpg

Cat No. GTX107938

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase-Activated Protein Kinase 5,Mapkap-K5,Mk-5,Mk5,Prak,Mapkapk5

Cellular Localization

Cytoplasm (Probable) , Nucleus

Background

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107938_IFA_19011507_508.jpg

GTX107938 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PRAK(GTX107938) antibody at 1:200 dilution.

GTX107938_WB_19011507_646.jpg

GTX107938 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:H1299
7.5% SDS PAGE
GTX107938 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf