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PRAK antibody [N3C3]

Anti-PRAK antibody [N3C3] used in Western Blot (WB). GTX107938
Anti-PRAK antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107938

Cat. No. GTX107938

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PRAK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950886

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase-activated protein kinase 5 , MAPKAP-K5 , MK-5 , MK5 , PRAK

Cellular Localization

Cytoplasm (Probable) , Nucleus

Background

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRAK antibody [N3C3] used in Western Blot (WB). GTX107938

GTX107938 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:H1299
7.5% SDS PAGE
GTX107938 diluted at 1:500

Anti-PRAK antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107938

GTX107938 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PRAK(GTX107938) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169