GeneTex
United States (US)

PRAME antibody [N1C2]

GTX106248_40562_WB_19011506_174.jpg

Cat No. GTX106248

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

IMR32 , U87-MG , MCF-7

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRAME. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CT130 antibody, MAPE antibody, OIP4 antibody, PRAME antibody, cancer/testis antigen 130 antibody, OIP-4 antibody, opa-interacting protein 4 antibody, Opa-interacting protein OIP4 antibody, preferentially expressed antigen of melanoma antibody, melanoma antigen preferentially expressed in tumors antibody, preferentially expressed antigen in melanoma antibody

Background

This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106248_40562_WB_19011506_174.jpg

GTX106248 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX106248 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf