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PRAME antibody

Anti-PRAME antibody used in Flow cytometry (FACS). GTX03381
Anti-PRAME antibody used in Western Blot (WB). GTX03381
Anti-PRAME antibody used in IHC (Paraffin sections) (IHC-P). GTX03381

Cat. No. GTX03381

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human
Package
400 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human PRAME.

Purification

Protein A purified followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

preferentially expressed antigen in melanoma , CT130 , MAPE , OIP-4 , OIP4

Cellular Localization

Nucleus , Cell membrane

Background

This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-PRAME antibody used in Flow cytometry (FACS). GTX03381

GTX03381 FACS Image

FACS analysis of 2% PFA-fixed HeLa cells using GTX03381 PRAME antibody. Acquisition of >10, 000 events was performed.
Green : Primary antibody
Blue : Isotype control
Permeabilization : 90% methanol for 10 min
Dilution : 1:25

Anti-PRAME antibody used in Western Blot (WB). GTX03381

GTX03381 WB Image

WB analysis of various sample lysates using GTX03381 PRAME antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : 293 whole cell lysate
Lane 4 : human testis tissue lysate
Lane 5 : human brain tissue lysate
Lane 6 : human kidney tissue lysate
Dilution : 1:2000
Loading : 20 μg per lane

Anti-PRAME antibody used in IHC (Paraffin sections) (IHC-P). GTX03381

GTX03381 IHC-P Image

IHC-P analysis of human testis tissue using GTX03381 PRAME antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399