Immunohistochemical analysis of paraffin-embedded human hepatoma, using PRAS40(GTX118391) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 antibody (GTX118391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 antibody (GTX118391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.