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PRAS40 antibody

Anti-PRAS40 antibody used in IHC (Paraffin sections) (IHC-P). GTX31882
Anti-PRAS40 antibody used in Western Blot (WB). GTX31882
Anti-PRAS40 antibody used in IHC (Paraffin sections) (IHC-P). GTX31882

Cat. No. GTX31882

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PRAS40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PRAS40 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PRAS40.The immunogen is located within amino acids 190 - 240 of PRAS40.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT1 substrate 1 , Lobe , PRAS40

Cellular Localization

Cytoplasm, cytosol

Background

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-PRAS40 antibody used in IHC (Paraffin sections) (IHC-P). GTX31882

GTX31882 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31882 PRAS40 antibody.
Working concentration : 2.5 μg/ml

Anti-PRAS40 antibody used in Western Blot (WB). GTX31882

GTX31882 WB Image

WB analysis of human brain tissue lysate using GTX31882 PRAS40 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-PRAS40 antibody used in IHC (Paraffin sections) (IHC-P). GTX31882

GTX31882 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31882 PRAS40 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399