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PRAS40 antibody

Anti-PRAS40 antibody used in IHC (Paraffin sections) (IHC-P). GTX87391
Anti-PRAS40 antibody used in Western Blot (WB). GTX87391

Cat. No. GTX87391

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PRAS40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human PRAS40 (207-256).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AKT1 substrate 1 , AKT1S1 , Lobe , PRAS40 , AKT1 substrate 1 (proline rich)

Cellular Localization

Cytoplasm,Cytosol

Background

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-PRAS40 antibody used in IHC (Paraffin sections) (IHC-P). GTX87391

GTX87391 IHC-P Image

IHC-P analysis of human brain tissue using GTX87391 PRAS40 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PRAS40 antibody used in Western Blot (WB). GTX87391

GTX87391 WB Image

WB analysis of 293 cell lysates using GTX87391 PRAS40 antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399