*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , 293T (100 nM Rapamycin, 30 min) , 293T (100nM Insulin, 30 min) , 293T (100 nM Rapamycin, 30 min)(100nM Insulin, 30 min)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.49 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide corresponding to residues around human PRAS40 (phospho Ser183). The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
AKT1 substrate 1 , Lobe , PRAS40
Cytoplasm , cytosol
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]