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PRAS40 (phospho Thr246) antibody

Orthogonal
Anti-PRAS40 (phospho Thr246) antibody used in Western Blot (WB). GTX133176
Anti-PRAS40 (phospho Thr246) antibody used in Western Blot (WB). GTX133176

Cat. No. GTX133176

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRAS40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

NIH3T3 , 293T , A431 , HepG2

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr246 of human PRAS40. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886855

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AKT1 substrate 1 , Lobe , PRAS40

Cellular Localization

Cytoplasm , cytosol

Background

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PRAS40 (phospho Thr246) antibody used in Western Blot (WB). GTX133176

GTX133176 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody (GTX133176) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRAS40 (phospho Thr246) antibody used in Western Blot (WB). GTX133176

GTX133176 WB Image

Orthogonal

Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody (GTX133176) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169