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PRAS40 (phospho Thr246) antibody

Anti-PRAS40 (phospho Thr246) antibody used in Western Blot (WB). GTX133176
Anti-PRAS40 (phospho Thr246) antibody used in Western Blot (WB). GTX133176

Cat No. GTX133176

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , NIH3T3

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human PRAS40 (phospho Thr246). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AKT1 substrate 1,Lobe,PRAS40

Cellular Localization

Cytoplasm , cytosol

Background

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-PRAS40 (phospho Thr246) antibody used in Western Blot (WB). GTX133176

GTX133176 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody (GTX133176) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRAS40 (phospho Thr246) antibody used in Western Blot (WB). GTX133176

GTX133176 WB Image

Untreated (–) and treated (+) NIH3T3 whole cell extracts (60 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Thr246) antibody (GTX133176) diluted at 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169