PRC1 antibody [16F2]

ICC/IF analysis of HeLa cells using GTX15796 PRC1 antibody [16F2]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

WB analysis of whole cell extracts (30 μg lysate) of PANC-1 (Lane 1), HCT 116 (Lane 2), C2C12 (Lane 3), HeLa (Lane 4), SH-SY5Y (Lane 5), K-562 (Lane 6) and Raji (Lane 7) using GTX15796 PRC1 antibody [16F2].
Dilution : 1-3 μg/ml

ICC/IF analysis of HeLa cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of A2058 Cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

ICC/IF analysis of U251 cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC