GeneTex
United States (US)

PRC1 antibody [16F2]

GTX15796_320_ICC-IF_19011519_746.jpg
GTX15796_321_ICC-IF_19011519_153.jpg
GTX15796_322_ICC-IF_19011519_899.jpg
GTX15796_1544_WB_19011519_553.jpg
GTX15796_323_ICC-IF_19011519_408.jpg

Cat No. GTX15796

Host Mouse
Clonality Monoclonal
Clone Name 16F2
Isotype IgG2b
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3 μg/ml
ICC/IF 1-2 μg/ml
IP Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant PRC1 protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Regulator Of Cytokinesis 1,Ase1,Prc1

Cellular Localization

Cytoplasm,Cytoskeleton,Midbody,Nucleus,Spindle pole

Background

This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES
GTX15796_320_ICC-IF_19011519_746.jpg

GTX15796 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15796 PRC1 antibody [16F2]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature

GTX15796_321_ICC-IF_19011519_153.jpg

GTX15796 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 °C

GTX15796_322_ICC-IF_19011519_899.jpg

GTX15796 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 °C

GTX15796_1544_WB_19011519_553.jpg

GTX15796 WB Image

WB analysis of whole cell extracts (30 µg lysate) of PANC-1 (Lane 1), HCT 116 (Lane 2), C2C12 (Lane 3), HeLa (Lane 4), SH-SY5Y (Lane 5), K-562 (Lane 6) and Raji (Lane 7) using GTX15796 PRC1 antibody [16F2].
Dilution : 1-3 µg/ml

GTX15796_323_ICC-IF_19011519_408.jpg

GTX15796 ICC/IF Image

ICC/IF analysis of U251 cells using GTX15796 PRC1 antibody [16F2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

Package List Price ($)
$ 319