*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Jurkat , Raji , Mouse testis
Rat, Bovine(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)
Recombinant protein encompassing a sequence within the N-terminus region of human PRC1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ASE1 antibody, MGC1671 antibody, MGC3669 antibody, PRC1 antibody, protein regulator of cytokinesis 1 antibody, protein regulating cytokinesis 1 antibody, anaphase spindle elongation 1 homolog antibody
This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
GTX115179 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using PRC1(GTX115179) antibody at 1:200 dilution.
GTX115179 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using PRC1(GTX115179) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX115179 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PRC1 antibody [N1N2], N-term (GTX115179) diluted at 1:1000.
GTX115179 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX115179 diluted at 1:1000