GeneTex
United States (US)

PRC1 antibody [N1N2], N-term

GTX115179_40254_IFA_18121410_951.jpg
GTX115179_40254_IHC_18121410_619.jpg
GTX115179_40254_20151105_WB_M_testis_18121410_418.jpg
GTX115179_WB_18121410_627.jpg

Cat No. GTX115179

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

Jurkat , Raji , Mouse testis

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PRC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ASE1 antibody, MGC1671 antibody, MGC3669 antibody, PRC1 antibody, protein regulator of cytokinesis 1 antibody, protein regulating cytokinesis 1 antibody, anaphase spindle elongation 1 homolog antibody

Background

This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115179_40254_IFA_18121410_951.jpg

GTX115179 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using PRC1(GTX115179) antibody at 1:200 dilution.

GTX115179_40254_IHC_18121410_619.jpg

GTX115179 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using PRC1(GTX115179) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115179_40254_20151105_WB_M_testis_18121410_418.jpg

GTX115179 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PRC1 antibody [N1N2], N-term (GTX115179) diluted at 1:1000.

GTX115179_WB_18121410_627.jpg

GTX115179 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
B: Raji
7.5% SDS PAGE
GTX115179 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf