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PRC1 antibody [N1N2], N-term

Anti-PRC1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115179
Anti-PRC1 antibody [N1N2], N-term used in Western Blot (WB). GTX115179
Anti-PRC1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115179
Anti-PRC1 antibody [N1N2], N-term used in Western Blot (WB). GTX115179

Cat. No. GTX115179

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

Jurkat , Raji , mouse testis

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PRC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619624

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein regulator of cytokinesis 1 , ASE1

Background

This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRC1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115179

GTX115179 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using PRC1(GTX115179) antibody at 1:200 dilution.

Anti-PRC1 antibody [N1N2], N-term used in Western Blot (WB). GTX115179

GTX115179 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
B: Raji
7.5% SDS PAGE
GTX115179 diluted at 1:1000

Anti-PRC1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115179

GTX115179 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using PRC1(GTX115179) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRC1 antibody [N1N2], N-term used in Western Blot (WB). GTX115179

GTX115179 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PRC1 antibody [N1N2], N-term (GTX115179) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169