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PRDM14 antibody

Anti-PRDM14 antibody used in Western Blot (WB). GTX54571
Anti-PRDM14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54571
Anti-PRDM14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54571
Anti-PRDM14 antibody used in ChIP assay (ChIP assay). GTX54571

Cat. No. GTX54571

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PRDM14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
ChIP assay 1:20 - 1:100
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human PRDM14

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PR/SET domain 14 , PFM11

Cellular Localization

Nucleus

Background

This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-PRDM14 antibody used in Western Blot (WB). GTX54571

GTX54571 WB Image

WB analysis of rat testis tissue lysate using GTX54571 PRDM14 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PRDM14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54571

GTX54571 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54571 PRDM14 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PRDM14 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54571

GTX54571 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54571 PRDM14 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PRDM14 antibody used in ChIP assay (ChIP assay). GTX54571

GTX54571 ChIP assay Image

ChIP analysis of 293 cell lysate using GTX54571 PRDM14 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399