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PRDM16 antibody

Cat No. GTX23936

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
100 μg ($329)

APPLICATION

Application Note

ELISA: Use at a dilution of 1/32000. WB: Use at a concentration of 2 μg/ml. Predicted molecular weight: 140.2 kDa. No signal obtained yet but low background observed in Raji, Daudi, MOLT-4 and Human Brain lysates Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

140 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: TSESGAFHPINHL, corresponding to amino acids 1264-1276 of Human PRDM 16 .

Purification

IgG fraction
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Pr/Set Domain 16 , Cmd1Ll , Kmt8F , Lvnc8 , Mel1 , Pfm13 , Prdm16

Cellular Localization

Nuclear

Background

Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Database

Research Area

REFERENCE

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REVIEW

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Package List Price ($)
$ 329