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PRDM4 antibody

Orthogonal
Anti-PRDM4 antibody used in IHC (Paraffin sections) (IHC-P). GTX115579
Anti-PRDM4 antibody used in Western Blot (WB). GTX115579
Anti-PRDM4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115579
Anti-PRDM4 antibody used in IHC (Paraffin sections) (IHC-P). GTX115579
Anti-PRDM4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115579

Cat. No. GTX115579

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRDM4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PRDM4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177791

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PR/SET domain 4 , PFM1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis. An elevated expression level of this gene has been observed in PC12 cells treated with nerve growth factor, beta polypeptide (NGF). This gene is located in a chromosomal region that is thought to contain tumor suppressor genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRDM4 antibody used in IHC (Paraffin sections) (IHC-P). GTX115579

GTX115579 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using PRDM4(GTX115579) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRDM4 antibody used in Western Blot (WB). GTX115579

GTX115579 WB Image

Orthogonal

PC-3 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRDM4 antibody (GTX115579) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRDM4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115579

GTX115579 ICC/IF Image

PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRDM4 protein stained by PRDM4 antibody (GTX115579) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-PRDM4 antibody used in IHC (Paraffin sections) (IHC-P). GTX115579

GTX115579 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using PRDM4(GTX115579) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRDM4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115579

GTX115579 ICC/IF Image

PRDM4 antibody detects PRDM4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRDM4 protein stained by PRDM4 antibody (GTX115579) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Cubenas-Potts C et al. Proteomics 2014; Identification of SUMO-2/3 modified proteins associated with mitotic chromosomes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169