APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse heart
Predict Reactivity
Human, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PRELP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885583
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
proline and arginine rich end leucine rich repeat protein , MST161 , MSTP161 , SLRR2A
Cellular Localization
Secreted , extracellular space , extracellular matrix
Background
The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX123359 WB Image
PRELP antibody detects PRELP protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PRELP antibody (GTX123359) diluted at 1:500.
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GTX123359 IHC-P Image
PRELP antibody detects PRELP protein at secreted on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PRELP antibody (GTX123359) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX123359 IHC-P Image
PRELP antibody detects PRELP protein at secreted on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PRELP antibody (GTX123359) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
REFERENCE
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REVIEW
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