GeneTex
United States (US)

PRELP antibody

GTX123359_41031_20150806_WB_M_heart_18121410_334.jpg
GTX123359_41031_IHC_M_18121410_183.jpg
GTX123359_41031_IHC_M_2_18121410_541.jpg

Cat No. GTX123359

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

mouse heart

Predict Reactivity

Human, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRELP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proline And Arginine Rich End Leucine Rich Repeat Protein,Mst161,Mstp161,Slrr2A,Prelp

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX123359_41031_20150806_WB_M_heart_18121410_334.jpg

GTX123359 WB Image

PRELP antibody detects PRELP protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PRELP antibody (GTX123359) diluted at 1:500.

GTX123359_41031_IHC_M_18121410_183.jpg

GTX123359 IHC-P Image

PRELP antibody detects PRELP protein at secreted on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
PRELP antibody (GTX123359) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX123359_41031_IHC_M_2_18121410_541.jpg

GTX123359 IHC-P Image

PRELP antibody detects PRELP protein at secreted on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PRELP antibody (GTX123359) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169