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PRELP antibody

Anti-PRELP antibody used in IHC (Paraffin sections) (IHC-P). GTX123359
Anti-PRELP antibody used in Western Blot (WB). GTX123359
Anti-PRELP antibody used in IHC (Paraffin sections) (IHC-P). GTX123359

Cat. No. GTX123359

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRELP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

mouse heart

Predict Reactivity

Human, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRELP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885583

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proline and arginine rich end leucine rich repeat protein , MST161 , MSTP161 , SLRR2A

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PRELP antibody used in IHC (Paraffin sections) (IHC-P). GTX123359

GTX123359 IHC-P Image

PRELP antibody detects PRELP protein at secreted on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PRELP antibody (GTX123359) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PRELP antibody used in Western Blot (WB). GTX123359

GTX123359 WB Image

PRELP antibody detects PRELP protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PRELP antibody (GTX123359) diluted at 1:500.

Anti-PRELP antibody used in IHC (Paraffin sections) (IHC-P). GTX123359

GTX123359 IHC-P Image

PRELP antibody detects PRELP protein at secreted on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PRELP antibody (GTX123359) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169