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PREX1 antibody

Overexpression
Anti-PREX1 antibody used in Western Blot (WB). GTX128036
Anti-PREX1 antibody used in Western Blot (WB). GTX128036
Anti-PREX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128036

Cat. No. GTX128036

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PREX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

186 kDa. ( Note )

Positive Control

human PREX1-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PREX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885687

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1 , P-REX1

Cellular Localization

Cytoplasm , cytosol , Cell membrane

Background

The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PREX1 antibody used in Western Blot (WB). GTX128036

GTX128036 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PREX1 antibody (GTX128036) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PREX1 antibody used in Western Blot (WB). GTX128036

GTX128036 WB Image

Overexpression

PREX1 antibody detects PREX1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human PREX1-transfected 293T cells
5 % SDS-PAGE
PREX1 antibody (GTX128036) dilution: 1:10000

Anti-PREX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128036

GTX128036 IHC-P Image

PREX1 antibody detects PREX1 protein at cytosol on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
PREX1 antibody (GTX128036) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169