GeneTex
United States (US)

PRG2 antibody [1.B.787]

Cat No. GTX14462

Host Mouse
Clonality Monoclonal
Clone Name 1.B.787
Isotype IgG1
Application WB, IHC-P, IHC-Fr, ELISA
Reactivity Human
APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 26.6 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

25 kDa. ( Note )

Specificity/Sensitivity

Specific for eosinophil major basic protein (14kD) irrespective of the stages of eosinophil activation and can be regarded as a ?pan-eosinophil? marker. Recognizes secreted pro-form of MBP (Pregnancy associated MBP, 23.8kD) and mature form found in the matrix of the eosinophil large specific granule (13.8kD).
This product does not react with Mouse,Rat,Horse.
PROPERTIES

Form

Liquid

Buffer

Preservative: 10mM Sodium Azide; Constituents: 1mg/ml BSA, PBS. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length protein (Human).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proteoglycan 2, Pro Eosinophil Major Basic Protein,Bmpg,Mbp,Mbp1,Prombp,Prg2

Cellular Localization

The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule (crystalloid core).

Background

PRG2 is a cytotoxin and helminthotoxin. Also induces noncytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.

Database

Package List Price ($)
$ 319