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PRG2 antibody [HL2547]

Recombinant antibody Orthogonal
Anti-PRG2 antibody [HL2547] used in IHC (Paraffin sections) (IHC-P). GTX638919
Anti-PRG2 antibody [HL2547] used in Western Blot (WB). GTX638919
Anti-PRG2 antibody [HL2547] used in Western Blot (WB). GTX638919

Cat. No. GTX638919

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2547

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRG2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human PRG2

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteoglycan 2, pro eosinophil major basic protein , BMPG , MBP , MBP1 , proMBP

Background

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. The encoded protein contains a peptide that displays potent antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria, and fungi. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-PRG2 antibody [HL2547] used in IHC (Paraffin sections) (IHC-P). GTX638919

GTX638919 IHC-P Image

PRG2 antibody [HL2547] detects secreted PRG2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
PRG2 stained by PRG2 antibody [HL2547] (GTX638919) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PRG2 antibody [HL2547] used in Western Blot (WB). GTX638919

GTX638919 WB Image

Orthogonal

Untreated (–) and treated (+) HL-60 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRG2 antibody [HL2547] (GTX638919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PRG2 antibody [HL2547] used in Western Blot (WB). GTX638919

GTX638919 WB Image

Orthogonal

Untreated (–) and treated (+) HL-60 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRG2 antibody [HL2547] (GTX638919) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations