Wild-type (WT) and PRKCSH knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRKCSH antibody [N1C1] (GTX101889) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PRKCSH antibody [N1C1] detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRKCSH protein stained by PRKCSH antibody [N1C1] (GTX101889) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX101889 diluted at 1:10000

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PRKCSH(GTX101889) antibody at 1:200 dilution.

PRKCSH antibody [N1C1] detects PRKCSH protein at cytoplasm on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
PRKCSH antibody [N1C1] (GTX101889) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min