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PRMT1 antibody [GT779]

Anti-PRMT1 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX630186
Anti-PRMT1 antibody [GT779] used in Western Blot (WB). GTX630186
Anti-PRMT1 antibody [GT779] used in Western Blot (WB). GTX630186
Anti-PRMT1 antibody [GT779] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630186
Anti-PRMT1 antibody [GT779] used in Western Blot (WB). GTX630186
Anti-PRMT1 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX630186

Cat. No. GTX630186

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT779

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PRMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Neuro2A , GL261 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRMT1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888191

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 1 , ANM1 , HCP1 , HRMT1L2 , IR1B4

Cellular Localization

Nucleus

Background

The PRMT1 gene encodes a protein arginine methyltransferase that functions as a histone methyltransferase specific for H4 (see MIM 602822).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PRMT1 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX630186

GTX630186 IHC-P Image

PRMT1 antibody [GT779] detects PRMT1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
PRMT1 stained by PRMT1 antibody [GT779] (GTX630186) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PRMT1 antibody [GT779] used in Western Blot (WB). GTX630186

GTX630186 WB Image

PRMT1 antibody [GT779] detects PRMT1 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
PRMT1 antibody [GT779] (GTX630186) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PRMT1 antibody [GT779] used in Western Blot (WB). GTX630186

GTX630186 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT1 antibody [GT779] (GTX630186) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PRMT1 antibody [GT779] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630186

GTX630186 ICC/IF Image

PRMT1 antibody [GT779] detects PRMT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRMT1 protein stained by PRMT1 antibody [GT779] (GTX630186) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-PRMT1 antibody [GT779] used in Western Blot (WB). GTX630186

GTX630186 WB Image

PRMT1 antibody [GT779] detects PRMT1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
PRMT1 antibody [GT779] (GTX630186) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PRMT1 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX630186

GTX630186 IHC-P Image

PRMT1 antibody [GT779] detects PRMT1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PRMT1 stained by PRMT1 antibody [GT779] (GTX630186) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations