Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT2 antibody [N1C2] (GTX103749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using PRMT2(GTX103749) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT2 antibody [N1C2] (GTX103749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PRMT2(GTX103749) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min