GeneTex
United States (US)

PRMT2 antibody [N1C2]

GTX103749_39995_IFA_19011506_844.jpg
GTX103749_WB_19011506_722.jpg
GTX103749_39995_IHC_19011506_793.jpg

Cat No. GTX103749

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HRMT1L1 antibody, MGC111373 antibody, PRMT2 antibody, PRMT2 alpha antibody, protein arginine N-methyltransferase 2 antibody, PRMT2 beta antibody, HMT1 hnRNP methyltransferase-like 1 antibody, histone-arginine N-methyltransferase PRMT2 antibody, PRMT2 gamma antibody, "HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1 antibody", protein arginine methyltransferase 2 antibody

Background

Probably methylates the guanidino nitrogens of arginyl residues in some proteins. May play a role in transcriptional coactivation.

Database

Research Area

DATA IMAGES
GTX103749_39995_IFA_19011506_844.jpg

GTX103749 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using PRMT2(GTX103749) antibody at 1:200 dilution.

GTX103749_WB_19011506_722.jpg

GTX103749 WB Image

Sample(30 µg whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX103749 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103749_39995_IHC_19011506_793.jpg

GTX103749 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PRMT2(GTX103749) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf