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PRMT2 antibody [N1C2]

Anti-PRMT2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103749
Anti-PRMT2 antibody [N1C2] used in Western Blot (WB). GTX103749
Anti-PRMT2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103749
Anti-PRMT2 antibody [N1C2] used in Western Blot (WB). GTX103749

Cat. No. GTX103749

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRMT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951450

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 2 , HRMT1L1

Background

Probably methylates the guanidino nitrogens of arginyl residues in some proteins. May play a role in transcriptional coactivation.

Database

Research Area

DATA IMAGES

Anti-PRMT2 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX103749

GTX103749 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PRMT2(GTX103749) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PRMT2 antibody [N1C2] used in Western Blot (WB). GTX103749

GTX103749 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT2 antibody [N1C2] (GTX103749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PRMT2 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103749

GTX103749 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using PRMT2(GTX103749) antibody at 1:200 dilution.

Anti-PRMT2 antibody [N1C2] used in Western Blot (WB). GTX103749

GTX103749 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRMT2 antibody [N1C2] (GTX103749) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169