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PRMT2 antibody

Anti-PRMT2 antibody used in Western Blot (WB). GTX103398
Anti-PRMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103398

Cat No. GTX103398

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all PRMT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein arginine methyltransferase 2 , HRMT1L1

Background

Probably methylates the guanidino nitrogens of arginyl residues in some proteins. May play a role in transcriptional coactivation.

Database

Research Area

DATA IMAGES

Anti-PRMT2 antibody used in Western Blot (WB). GTX103398

GTX103398 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX103398 diluted at 1:3000

Anti-PRMT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103398

GTX103398 ICC/IF Image

PRMT2 antibody detects PRMT2 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRMT2 protein stained by PRMT2 antibody (GTX103398) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169