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PRMT3 antibody

Overexpression
Anti-PRMT3 antibody used in Western Blot (WB). GTX116478
Anti-PRMT3 antibody used in Immunoprecipitation (IP). GTX116478
Anti-PRMT3 antibody used in Western Blot (WB). GTX116478
Anti-PRMT3 antibody used in Western Blot (WB). GTX116478

Cat. No. GTX116478

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PRMT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

U87-MG , Mouse brain , PRMT3-transfected 293T

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRMT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615505

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein arginine methyltransferase 3 , HRMT1L3

Cellular Localization

Cytoplasm

Background

Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PRMT3 antibody used in Western Blot (WB). GTX116478

GTX116478 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT3 antibody (GTX116478) diluted at 1:1000.

Anti-PRMT3 antibody used in Immunoprecipitation (IP). GTX116478

GTX116478 IP Image

PRMT3 antibody immunoprecipitates PRMT3 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of PRMT3 protein by 4 μg PRMT3 antibody (GTX116478)
7.5 % SDS-PAGE
The immunoprecipitated PRMT3 protein was detected by PRMT3 antibody (GTX116478) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-PRMT3 antibody used in Western Blot (WB). GTX116478

GTX116478 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX116478 diluted at 1:1000

Anti-PRMT3 antibody used in Western Blot (WB). GTX116478

GTX116478 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX116478 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169